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KMID : 0358420140570060501
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Korean Journal of Obstetrics and Gynecology
2014 Volume.57 No. 6 p.501 ~ p.506
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Salinomycin inhibited cell proliferation and induced apoptosis in human uterine leiomyoma cells
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Lee Hyun-Gyo
Lee Ji-Min
Shin So-Jin
Kwon Sang-Hoon
Lee Gi-Su
Song Chang-Ho
Choi Eun-Som
Cha Soon-Do
Cho Chi-Heum
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Abstract
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Objective: The aim of this study was to investigate the anti-proliferative effect of the salinomycin in cell proliferation and apoptosis in primary cultured human uterine leiomyoma cells.
Methods: Cell viability was measured by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Caspase-3 activity assay and DNA fragmentation assay were performed to determine the effect of apoptosis. The expression of apoptosis regulatory-related proteins was evaluated by western blot.
Results: The cell viability and proliferation of uterine leiomyoma cells were significantly reduced by salinomycin treatment in a dose-dependent manner. DNA fragmentation assay results showed apoptotic cell death after salinomycin incubation. Salinomycin activated caspase-3, -8, and -9, causing apoptosis in uterine leiomyoma cells. Down-regulation of Bcl-2, XIAP, and FLIP with a concomitant increase in Bax, Fas, and DR5 were observed.
Conclusion: These results provided the first evidence that salinomycin induce both intrinsic and extrinsic apoptosis. Therefore, salinomycin may be a promising chemopreventive and therapeutic agent against human uterine leiomyoma.
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KEYWORD
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Apoptosis, Extrinsic, Intrinsic, Leiomyoma, Salinomycin
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